Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (2023)

Product Name

Acrolein Antibody

Description

Mouse Anti-Acrolein conjugated protein Monoclonal IgG1

Species Reactivity

Species Independent

Applications

WB, ICC/IF, FACS, FCM, ELISA

Antibody Dilution

WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.

Host Species

Mouse

Immunogen

Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).

Concentration

1 mg/ml

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated

Dylight 488

Overview:

High fluorescence yield
High photostability
Less pH-sensitive
Excellent batch-to-batch reproducibility
Stringently QC tested
Molecular weight:1011 g/mol

Dylight 488 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (1)

Optical Properties:

λ_ex= 493 nm

λ_em = 518 nm

ε_max = 7.0×10⁴

Laser= 488 nm

APC/Cy7

Overview:

High quantum yield
Excellent batch-to-batch reproducibility
Stringently QC tested

APC-Cy7 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (2)

Optical Properties:

λ_ex = 652 nm

λ_em = 790 nm

Laser = 594 or 633 nm

Dylight 350

Overview:

High fluorescence intensity
High photostability
Less pH-sensitive
Excellent solubility in water
Stringently QC tested
Excellent batch-to-batch reproducibility
Molecular weight:874 g/mol

Dylight 350 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (3)

Optical Properties:

λ_ex= 353 nm

λ_em = 432 nm

ε_max = 1.5×10⁴

Dylight 405

Overview:

High fluorescence intensity
High photostability
Less pH-sensitive
Excellent batch-to-batch reproducibility
Stringently QC tested
Molecular weight:793 g/mol

Dylight 405 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (4)

Optical Properties:

λ_ex= 400 nm

λ_em = 420 nm

ε_max = 3.0×10⁴

Laser = 405 nm

Dylight 594

Overview:

High fluorescence yield
High photostability
Less pH-sensitive
Excellent batch-to-batch reproducibility
Stringently QC tested
Molecular weight:1078 g/mol

Dylight 594 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (5)

Optical Properties:

λ_ex= 593 nm

λ_em = 618 nm

ε_max = 8.0×10⁴

Laser= 526 nm

Dylight 633

Overview:

High fluorescence yield
High photostability
Less pH-sensitive
Excellent batch-to-batch reproducibility
Stringently QC tested
Molecular weight:1066 g/mol

Dylight 633 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (6)

Optical Properties:

λ_ex= 638 nm

λ_em = 658 nm

ε_max = 1.7×10⁵

Laser= 633nm

PerCP

Overview:

Peridinin-Chlorophyll-Protein Complex
Small phycobiliprotein
Isolated from red algae
Large stokes shift (195 nm)
Molecular Weight: 35 kDa

PerCP Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (7)

Optical Properties:

λ_ex= 482 nm

λ_em = 677 nm

ε_max = 1.96 x 10⁶

Laser= 488 nm

PE/ATTO 594

PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation

PE/ATTO 594 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (8)

Optical Properties:

λ_ex= 535 nm

λ_em= 627nm

Laser=488 to 561 nm

FITC (Fluorescein)

Overview:

Excellent fluorescence quantum yield
High rate of photobleaching
Good solubility in water
Broad emission spectrum
pH dependent spectra
Molecular formula: C₂₀H₁₂O₅
Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (9)

Optical Properties:

λ_ex= 494nm

λ_em = 520nm

ε_max = 7.3×10⁴

Φ_f =0.92

τ_fl= 5.0 ns

Brightness =67.2

Laser=488nm

Filter set=FITC

ATTO 700

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 575 g/mol

ATTO 700 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (10)

Optical Properties:

λ_ex= 700nm

λ_em = 719 nm

ε_max = 1.25×10⁵

Φ_f =0.25

τ_fl= 1.6 ns

Brightness =31.3

Laser= 676 nm

Filter set= Cy®5.5

ATTO 680

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 631 g/mol

ATTO 680 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (11)

Optical Properties:

λ_ex= 680 nm

λ_em = 700nm

ε_max = 1.25×10⁵

Φ_f =0.30

τ_fl= 1.7 ns

Brightness = 37.5

Laser= 633– 676 nm

Filter set= Cy®5.5

ATTO 655

Overview:

High fluorescence yield
High thermal and photostability
Excellent ozone resistance
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 634 g/mol

ATTO 655 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (12)

Optical Properties:

λ_ex= 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f =0.30

τ_fl= 1.8 ns

Brightness =37.5

Laser= 633– 647 nm

Filter set= Cy®5

ATTO 633

Overview:

High fluorescence yield
High thermal and photostability
Moderately hydrophilic
Good solubility in polar solvents
Stable at pH 4 – 11
Cationic dye, perchlorate salt
Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (13)

Optical Properties:

λ_ex= 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f =0.64

τ_fl= 3.2 ns

Brightness = 83.2

Laser= 633nm

Filter set= Cy®5

ATTO 594

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 1137 g/mol

ATTO 594 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (14)

Optical Properties:

λ_ex= 601nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f =0.85

τ_fl= 3.5 ns

Brightness = 102

Laser= 594 nm

Filter set= Texas Red®

ATTO 565

Overview:

High fluorescence yield
High thermal and photostability
Good solubility in polar solvents
Excellent solubility in water
Very little aggregation
Rhodamine dye derivative
Molar Mass: 611 g/mol

ATTO 565 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (15)

Optical Properties:

λ_ex= 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f =0.9

τ_fl= 3.4 n

Brightness = 10

Laser= 532 nm

Filter set=TRITC

ATTO 488

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 804 g/mol

ATTO 488 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (16)

Optical Properties:

λ_ex= 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f =0.80

τ_fl= 4.1 ns

Brightness =72

Laser=488nm

Filter set=FITC

ATTO 390

Overview:

High fluorescence yield
Large Stokes-shift (89 nm)
Good photostability
Moderately hydrophilic
Good solubility in polar solvents
Coumarin derivate, uncharged
Low molar mass: 343.42 g/mol

ATTO 390 Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (17)

Optical Properties:

λ_ex= 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f =0.90

τ_fl= 5.0 ns

Brightness =21.6

Laser= 365 or 405 nm

APC (Allophycocyanin)

Overview:

High quantum yield
Large phycobiliprotein
6 chromophores per molecule
Isolated from red algae
Molecular Weight: 105 kDa

APC Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (18)

Optical Properties:

λ_ex= 650 nm

λ_em = 660 nm

ε_max = 7.0×10⁵

Φ_f =0.68

Brightness =476

Laser= 594 or 633nm

Filter set= Cy®5

Streptavidin

Properties:

Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
Molecular weight: 53 kDa
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target
Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors
Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
High turnover rate enables rapid generation of a strong signal
44 kDa glycoprotein
Extinction coefficient: 100 (403 nm)
Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
Molecular weight: 140 kDa
Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

R-PE (R-Phycoerythrin)

Overview:

Broad excitation spectrum
High quantum yield
Photostable
Member of the phycobiliprotein family
Isolated from red algae
Excellent solubility in water
Molecular Weight: 250 kDa

R-PE Datasheet

Anti-Acrolein Antibody [2H2] MOLECULAR SIGNATURE® (20)

Optical Properties:

λ_ex= 565 nm

λ_em = 575 nm

ε_max = 2.0×10⁶

Φ_f =0.84

Brightness = 1.68 x 10³

Laser=488 to 561 nm

Filter set=TRITC

Field of Use

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20ºC, Conjugated antibodies should be stored at 4ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Protein G Purified
Clonality	Monoclonal
Clone Number	2H2
Isotype	IgG1
Cite This Product	StressMarq Biosciences Cat# SMC-504, RRID: AB_2702711
Certificate of Analysis	A 1:1000 dilution of SMC-504 was sufficient for detection of Acrolein in 2 µg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Biological Description

Alternative Names	Acrolein modified protein Antibody, Acrolein conjugated protein Antibody, 2-Propen-1-one Antibody, 2-propenal Antibody, Acraldehyde Antibody, Acrolein Antibody, Acrylic aldehyde Antibody, Protein-bound Acrolein Antibody
Research Areas	Alzheimer's Disease, Cancer, Lipid peroxidation, Neurodegeneration, Neuroscience, Oxidative Stress
Scientific Background	Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Acrolein is an electrophilic conjugated aldehyde that is a terminal product of lipid peroxidation. Acrolein is highly mutagenic and reacts with nucleophilic functional groups in DNA and proteins such as cysteine, histidine, and lysine residues (1).
References	1. Takabe, W. et al. (2001) Carcinogenesis. 22 (6): 935-941

Product Images

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (SMC-504). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (SMC-504) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) – Untreated. (B,D,F,H) – Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (SMC-504). Lane 1: Molecular Weight Ladder (MW). Lane 2: Acrolein-BSA (0.5 µg). Lane 3: Acrolein-BSA (2.0 µg). Lane 4: BSA (0.5 µg). Lane 5: BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (SMC-504) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (SMC-504). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (SMC-504) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.

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